IGF1 Receptor (IGF1R) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7G11]

CAT#: AM00072BT-N

IGF1 Receptor (IGF1R) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 7G11, Biotin

Conjugation: Unconjugated Biotin



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CNY 6,798.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

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Specifications

Product Data
Clone Name 7G11
Applications WB
Recommend Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Icluded Positive Control: Cell lysate from untreated A431 cells (See Protocols for more details).
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH.
Specificity This antibody specifically recognizes the C-terminus of IGF1 Receptor.
Formulation PBS
Label: Biotin
State: Liquid purified IgG fraction.
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Biotin
Storage Condition Store the antibody (aliquote in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size 97 kDa
Gene Name insulin like growth factor 1 receptor
Background The IGF1 receptor (IGF1R) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The IGF1 receptor is activated upon binding of the peptide hormones IGF1 and 2, leading to autophosphorylation of tyrosine residue 1131, 1135, and 1136 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residue 950 and 1316 regulate the assembly of signal transduction complexes.
Synonyms IGF-I receptor, IGF1 Receptor
Note Protocol: Positive Control Cell Lysate A431 Untreated

Formulation: Lyophilized cell lysate from serum starved A431 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.

Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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