OriGene 抗体特异性验证

产品特异性是抗体特性非常重要的一个方面。OriGene 已经采用多种方式来进行产品特异性验证

验证方式

  • 根据预测分子量大小，通过Western blot进行分析
  • 独立抗体验证
  • 10k 蛋白芯片验证
  • Western blot使用敲除细胞系（KO）验证

验证数据示例

Specificity Validation for OriGene antibody Cat. No. UM500036
anti-her2 validation with over expression and endogenous lysates

Figure 1. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using human Her2 overexpression cell lysates and endogenous cell line lysates. UM500036 can recognize the specific Her2 protein at predicted size (~140KDa) in overexpression cell ysate (lane 1 ) and endogenous lysates prepared from Hela, A549 and MCF7 cell lines. The specificity is further proved by using an independent antibody (anti-DDK antibody in the middle) against the same recombinant overexpressed Her2 protein.

anti-her2 validation with 10k protein chip

Figure 2. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using 10k protein chip technology. UM500036 shows no cross-reactivity with other protein on the tested protein microarray containing >10,000 overexpression lysates for different human proteins.

anti-her2 validation with-ko cell ysate

Figure 3. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using Her2 knockout cell lysate. UM500036 recognizes the specific Her2 protein at correct size in wild type Hela cell lysate (WT lane), and shows cross-reactivity with any other protein in the double knockout cell lysates (KO lane).
WT: parental wild type Hela cell lysate
KO: Her2 double knockout Hela cell lysate

抗体相关资料