Purified recombinant protein of Mouse keratin 8 (Krt8), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Proteins
Cytokeratin 8 bovine protein, 0.1 mg
| Protein Source | Liver |
Cytokeratin 8 human recombinant protein, 0.1 mg
| Expression Host | E. coli |
Cytokeratin 8 human recombinant protein, 0.25 mg
| Expression Host | E. coli |
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Expression Hosts
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- 10 mM Tris-HCl (4)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (4)
- the solution can be further dialyzed against PBS (phosphate buffered saline (4)
- 2 mM dithiothreitol (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (2) - e.g. Dulbecco's PBS). (2)
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e.g. Dulbeccos PBS).
See References 2 and 3 for more details. (2) - pH 7.4). For immunization purposes (2)
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pH 7.4).
For Immunization purposes (2)
