Proteins

Proteins

Native Proteins

Recombinant Proteins

Chicken

Human

DES

E. coli

10 mM Tris-HCl

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

e.g. Dulbecco's PBS).

pH 7.4). For immunization purposes

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

Stomach

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