Iba1 (149-161) goat polyclonal antibody, Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
| Applications | ELISA, IHC, WB |
| Reactivities | Hamster, Human, Monkey, Mouse, Porcine, Rat, Bovine, Equine, Goat, Rabbit, Pig |





![Western blot analysis of various lysates using AIF1/IBA1 Rabbit pAb ([TA373377]) at 1:5000 dilution incubated overnight at 4℃.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25 μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Negative control (NC): HeLa<br/>Exposure time: 10s.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/121/ta373377-1-w.jpg?browse)
![Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using AIF1/IBA1 Rabbit pAb ([TA373377]) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/121/ta373377-2-h.jpg?browse)
![Immunohistochemistry analysis of paraffin-embedded Human tonsil using AIF1/IBA1 Rabbit pAb ([TA373377]) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/121/ta373377-3-h.jpg?browse)




![Immunofluorescence analysis of paraffin-embedded rat brain using AIF1/IBA1 Rabbit pAb ([TA373376]) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/121/ta373376-2-f.jpg?browse)

