pCMV6-AC-FP635 Mammalian Expression Vector

Specifications

Product Data
C-Tag	TurboFP635
E. coli Selection	Ampicillin (100 ug/mL)
Cell Selection	Neomycin
Features	ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal TurboFP635 tag. TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close or mPlum. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays. TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for whole body imaging, cell and organelle labeling, and for tracking the promoter activity in auto-fluorescent tissues. If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal FP635-tagging vector, PS100046. V2_F Primer can be used to sequence the target sequence after cloning. TurboFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites

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