pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro Lentiviral Gene Expression Vector

Specifications

Product Data
C-Tag	Myc-DDK
N-Tag	HA
E. coli Selection	Chloramphenicol (34 ug/mL)
Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment. The ORF cloned in this vector can be expressed as a tagged protein with N-terminal HA tag and C-terminal Myc-DDK tag. IRES-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphenicol (34 ug/ml) EF51 Primer can be used to sequence the target sequence after cloning. IRES_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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