pCas-Guide-Puro-CRISPRi gene interference vector

CAT＃: GE100083

pCas-Guide-Puro-CRISPRi Vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with Puro selection marker, dCas9-KRAB-MeCP2 and gRNA cloning site.

Datasheet
SDS
Vector Sequence

CNY 4,560.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

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TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

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CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

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Specifications

Product Data
Features
  • All-in-one CRISPRi vector system
  • gRNA driven by U6 promoter (after target sequence cloned)
  • dCas9-KRAB-MeCP2 fusion protein expression by CMV promoter
  • CF3 primer can be used to sequence target sequence after cloning
  • Contains puromycin resistance gene under SV40 promoter
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this Vectors has been cited in the following citations:
TGF-β signaling and Creb5 cooperatively regulate Fgf18 to control pharyngeal muscle development ,null, eLife ,PubMed ID 36542062

Documents

Product Manuals
FAQs
SDS
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