pT7-Guide-IVT

CAT＃: GE100025

pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)

Datasheet
SDS
Vector Sequence

CNY 3,710.00

货期*
现货
规格
    • 10 ug
Cited in 4 publications.

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Specifications

Product Data
Function CRISPR/Cas9 T7 Vectors
Features

T7 driven gRNA cloning vector

  • gRNA can be produced via in vitro transcription after the target sequence being cloned
  • BsmB I sites are used for target sequence cloning
  • For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
  • M13 forward sequencing primer can be used to sequence the target sequence after cloning.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

The use of this Vectors has been cited in the following citations:
Two CRISPR/Cas9-mediated methods for targeting complex insertions, deletions, or replacements in mouse ,Pineault, K;Novoa, A;Lozovska, A;Wellik, D;Mallo, M;, MethodsX ,PubMed ID 31667107 [pT7-Guide-IVT]
PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction ,Zhou, W;Ma, D;Sun, AX;Tran, HD;Ma, DL;Singh, BK;Zhou, J;Zhang, J;Wang, D;Zhao, Y;Yen, PM;Goh, E;Tan, EK;, Hum. Mol. Genet. ,PubMed ID 30496485 [pT7-Guide-IVT]
MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping ,null, Nature immunology ,PubMed ID 28759003
MICU1 Serves as a Molecular Gatekeeper to Prevent In Vivo Mitochondrial Calcium Overload ,Liu, JC;Liu, J;Holmström, KM;Menazza, S;Parks, RJ;Fergusson, MM;Yu, ZX;Springer, DA;Halsey, C;Liu, C;Murphy, E;Finkel, T;, Cell Rep ,PubMed ID 27477272 [CRISPR]

Documents

Product Manuals
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SDS
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