CYRIB Human siRNA Oligo Duplex (Locus ID 51571)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_001256763, NM_001330612, NM_016623, NR_046359, NR_046360, NR_046361, NM_001353242, NM_001353243, NM_001353244, NM_001353245, NM_001353246, NM_001353247, NM_001353248, NM_001353249, NM_001353250, NM_001353251, NM_001353252, NM_001353253, NM_001353254, NM_001353255, NM_001353256, NM_001353257, NM_001353258, NM_001353259, NM_001353260, NM_001353261, NM_001353262, NM_001353263, NM_001353264, NM_001353265, NM_001353266, NM_001353267, NM_001353268, NM_001353269, NM_001353270, NM_001353271, NM_001353272, NM_001353273, NM_001353274, NM_001353275, NM_001353276, NM_001353277, NM_001353278, NM_001353279, NM_001353280, NM_001353281, NM_001353282, NM_001353283, NM_001353284, NM_001353285, NM_001353286, NM_001353287, NM_001353288, NM_001353289, NM_001353290, NM_001353291, NM_001353292, NM_001353293, NM_001353294, NM_001353295, NM_001353296, NM_001353297, NM_001353298, NM_001353299, NM_001353300, NM_001353301, NM_001353302, NM_001353303, NM_001353304, NM_001353305, NM_001353306, NM_001353307, NM_001353308, NM_001353309, NM_001353310, NM_001353311, NM_001353312, NM_001353313, NM_001353314, NM_001353315, NM_001353316
Synonyms	BM-009; CYRI; CYRI-B; FAM49B; L1
Components	FAM49B (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 51571) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed:31285585, PubMed:30250061). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (PubMed:30250061). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed:31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (By similarity). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed:29059164).[UniProtKB/Swiss-Prot Function]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.