Fam65b Mouse shRNA Plasmid (Locus ID 193385)

Specifications

Product Data
Product Name	Fam65b Mouse shRNA Plasmid (Locus ID 193385)
Locus ID	193385
UniProt ID	Q80U16
Synonyms	1700108N18Rik; 6330500D04Rik; AI225904; E430013J17Rik; Fam65b; RP23-461O24.1
Vector	pRS
Format	Retroviral plasmids
Kit Components	Fam65b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 193385). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001080381, NM_001286100, NM_001286101, NM_029679, NM_178658, NM_178658.1, NM_178658.2, NM_178658.3, NM_178658.4, NM_178658.5, NM_001080381.1, NM_029679.1, NM_029679.2, NM_001286101.1, NM_001286100.1, BC059179, BC060202
Summary	Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization (PubMed:25588844, PubMed:27269051). Plays a role in fetal mononuclear myoblast differentiation by promoting filopodia and myotube formation (PubMed:17150207). Maintains naive T lymphocytes in a quiescent state and prevents chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (By similarity). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion (PubMed:25588844). Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear (PubMed:27269051). Plays a role for maintaining the structural organization of the basal domain of stereocilia (PubMed:27269051). Involved in mechanosensory hair cell function (PubMed:27269051). Required for normal hearing (PubMed:27269051).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.