Hnrnpc Mouse shRNA Plasmid (Locus ID 15381)

Specifications

Product Data
Product Name	Hnrnpc Mouse shRNA Plasmid (Locus ID 15381)
Locus ID	15381
UniProt ID	Q9Z204
Synonyms	AL022939; D14Wsu171e; hnrnp-C; hnRNPC1; hnRNPC2; Hnrpc; Hnrpc1; Hnrpc2
Vector	pRS
Format	Retroviral plasmids
Kit Components	Hnrnpc - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 15381). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC004706, BC095922, NM_001170981, NM_001170982, NM_001170983, NM_001170984, NM_016884, NM_001360172, NM_001360173, NM_001360174, NM_001360175, NM_001360176, NM_001360177, NM_001360178, NM_001360179, NM_001360180, NM_001360181, NM_001360182, NM_001360183, NM_001360184, NM_001170984.1, NM_001170982.1, NM_016884.1, NM_016884.2, NM_016884.3, NM_001170981.1, NM_001170983.1, NM_001170981.2, NM_001170982.2, NM_016884.4
Summary	Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3' UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.