TBC1D5 Human shRNA Plasmid Kit (Locus ID 9779)

Specifications

Product Data
Product Name	TBC1D5 Human shRNA Plasmid Kit (Locus ID 9779)
Locus ID	9779
UniProt ID	Q92609
Synonyms	KIAA0210
Vector	pRS
Format	Retroviral plasmids
Kit Components	TBC1D5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9779). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001134380, NM_001134381, NM_014744, NM_001349073, NM_001349074, NM_001349075, NM_001349076, NM_001349077, NM_001349078, NM_001349079, NM_001349080, NM_001349081, NM_001349082, NM_001349083, NM_001349084, NM_001349085, NM_001349086, NM_001349087, NM_001349088, NM_001349089, NM_001349090, NM_001349091, NM_014744.1, NM_014744.2, NM_001134380.1, NM_001134381.1, BC013145, BM976960, NM_001134381.2
Summary	May act as a GTPase-activating protein (GAP) for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity (PubMed:19531583, PubMed:20923837). Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy (PubMed:22354992). May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (PubMed:24603492).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.