TMC6 Human shRNA Plasmid Kit (Locus ID 11322)

Specifications

Product Data
Product Name	TMC6 Human shRNA Plasmid Kit (Locus ID 11322)
Locus ID	11322
UniProt ID	Q7Z403
Synonyms	EV1; EVER1; EVIN1; LAK-4P; lnc; TNRC6C-AS1
Vector	pRS
Format	Retroviral plasmids
Kit Components	TMC6 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 11322). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001127198, NM_001321185, NM_007267, NM_007267.1, NM_007267.2, NM_007267.3, NM_007267.4, NM_007267.5, NM_007267.6, NM_007267.7, NM_001127198.1, NM_001127198.2, BC023597, BC023597.2, BC007766, BC018346, BC035648, NM_001127198.5
Summary	Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 10 transmembrane domains and 2 leucine zipper motifs. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.