Non Neuronal Enolase (ENO1) Human shRNA Plasmid Kit (Locus ID 2023)

Specifications

Product Data
Product Name	Non Neuronal Enolase (ENO1) Human shRNA Plasmid Kit (Locus ID 2023)
Locus ID	2023
UniProt ID	P06733
Synonyms	ENO1L1; HEL-S-17; MPB1; NNE; PPH
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	ENO1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2023). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001201483, NM_001428, NM_001353346, NM_001428.1, NM_001428.2, NM_001428.3, NM_001201483.1, BC022545, BC022545.1, BC001810, BC004325, BC004458, BC009218, BC009912, BC011130, BC015641, BC021166, BC027725, BC050642, BC113034, NM_001428.5, NM_001201483.4
Summary	This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.