Recombinant IL-5 (Interleukin-5), Mouse

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 13.93 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant mouse IL-5 is > 5 x 10⁶ IU/mg.
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	P04401
Synonyms	EDF, BCDFII, TRF
Summary	Interleukin 5 (IL-5) is a 45-60 kDa homodimeric cytokine that functions principally in the eosinophil arm of the Th2 response. It is a member of the helical bundle family of cytokines, along with IL-3, IL-4, and granulocyte macrophage-colony stimulating factor (GM-CSF).

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