Recombinant IL-31 (Interleukin-31), Human

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MSHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT with polyhistidine tag at the C-terminus.
Tag	His Tag (C-term)
Predicted MW	The protein has a calculated MW of 16.78 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Purity	>98% as determined by SDS-PAGE.
Buffer	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
Applications	Cell culture
Storage	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Measured by its ability to induce A549 cells proliferation. The ED₅₀ for this effect is < 40 ng/mL
Endotoxin	<0.1 EU per 1 μg of the protein by the LAL method.
Reference Data
UniProt ID	Q6EBC2
Synonyms	IL31
Summary	Interleukin 31 (IL-31) is an 18 kDa cytokine with 142 amino acid residues. IL-31 is mainly secreted from activated CD4+ T cells and binds to a receptor called IL-31RA. Upon binding to 31RA, IL-31 activates several signal pathways like MAPK, PI3K/AKT, and Jak/STAT pathways. It is critical in regulating many biological functions, such as cell proliferation, hematopoiesis, induction of cytokines, inflammation, and immune response.

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