Thpo Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Protein Source	Human
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Ser22-Thr356
Tag	N-His
Buffer	Lyophilized from a 0.2 um filtered solution of PBS,2mM EDTA,pH7.4.
Note	Recombinant Mouse Thrombopoietin is produced by our Mammalian expression system and the target gene encoding Ser22­Thr356 is expressed with a 6His tag at the N-terminus.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Stability	12 months from date of despatch
Bioactivity	Measured in a cell proliferation assay using MO7E human megakaryocytic leukemic cells.The ED50 for this effect is 0.60 ng/ml.
Endotoxin	<0.05EU/μg as determined by LAL test.
Reference Data
Locus ID	21832
UniProt ID	P40226
Synonyms	Thrombopoietin;C-mpl ligand;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor;Myeloproliferative leukemia virus oncogene ligand;THPO
Summary	Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets.Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

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