Il2 (NM_053836) Rat Recombinant Protein

Specifications

Product Data
Species	Rat
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Rat IL-2, the region of Ala21-Met153, from gene Accession# NM_053836
Tag	Tag Free
Predicted MW	15.4 kDa
Concentration	lot specific
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Buffer	5 mM NaH2PO4, 5 mM citric acid, 150 mM NaCl, pH 4.0
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.102 - 0.295 ng/ml, corresponding to a specific activity of 9.8 - 3.39 x 106 units/mg, determined by the dose dependent stimulation of CTLL-2 cells.
Endotoxin	Less than 0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method
Reference Data
RefSeq	NP_446288
Locus ID	116562
UniProt ID	P17108
Refseq Size	740
Cytogenetics	2q25
Refseq ORF	465
Summary	cytokine produced by T-cells in response to antigen or mitogen stimulation [RGD, Feb 2006]

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