Il12b (NM_008352) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	Sf9
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Secreted protein (p40: Accession# NM_008352, p35: Accession# NM_008351
Tag	Tag Free
Predicted MW	57.5 kDa
Concentration	lot specific
Purity	95%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.10 to 0.20 ng/ml, corresponding to a specific activity of 1.0 - 0.5 x 10^7 units/mg, determined by the dose dependent stimulation of IFNγ production by mouse splenocytes.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_032378
Locus ID	16160
UniProt ID	P43432
Refseq Size	1951
Cytogenetics	11 25.94 cM
Refseq ORF	1005
Synonyms	Il-12b; Il-12p40; Il12p40; p40
Summary	This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]

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