Il10 (NM_010548) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Mouse IL-10, the region of Ser19-Ser178, from gene Accession# NM_010548, with a Cys149Tyr substitution
Tag	Tag Free
Predicted MW	18.9 kDa
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	20 mM Tris pH 7.5
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 1- 2 ng/ml, corresponding to a specific activity of 1- 0.5 x 10^6 units/mg, determined by the dose dependent stimulation MC/9 cell proliferation.
Endotoxin	Less than 0.01 ng per ug protein as determined by the LAL method
Reference Data
RefSeq	NP_034678
Locus ID	16153
UniProt ID	P18893
Refseq Size	1306
Cytogenetics	1 56.89 cM
Refseq ORF	534
Synonyms	CSIF; If2a; IL-; Il-10
Summary	This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]

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