HAPLN1 (16-354, His-Tag) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) DHLSDNYTLD HDRAIHIQAE NGPHLLVEAE QAKVFSHRGG NVTLPCKFYR DPTAFGSGIH KIRIKWTKLT SDYLKEVDVF VSMGYHKKTY GGYQGRVFLK GGSDSDASLV ITDLTLEDYG RYKCEVIEGL EDDTVVVALD LQGVVFPYFP RLGRYNLNFH EAQQACLDQD AVIASFDQLY DAWRGGLDWC NAGWLSDGSV QYPITKPREP CGGQNTVPGV RNYGFWDKDK SRYDVFCFTS NFNGRFYYLI HPTKLTYDEA VQACLNDGAQ IAKVGQIFAA WKILGYDRCD AGWLADGSVR YPISRPRRRC SPTEAAVRFV GFPDKKHKLY GVYCFRAYN
Tag	His-Tag
Predicted MW	39.3kDa (345aa)
Concentration	0.25mg/ml (determined by Absorbance at 280nm)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Applications	SDS-PAGE, Bioactivity
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Measured by its binding ability in a functional ELISA with Hyaluronic acid. The ED50 range ≤ 1 ug/ml.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_001875.1
Locus ID	1404
UniProt ID	P10915
Synonyms	hyaluronan and proteoglycan link protein 1, CRT1, CRTL1, HPLN1, Cartilage-linking protein 1, Proteoglycan link protein
Summary	HAPLN1, also known as CRTL1, is a member of the hyaladherin family. This protein contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. It involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. It stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Also, It has been shown to interact with Versican, two extracellular matrix components essential for cardiac development. Recombinant human HAPLN1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

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