Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) E296-G580
Tag	C-His
Predicted MW	The protein has a predicted molecular weight of 33.7 kDa and migrates at approximately 35-45 kDa on SDS-PAGE with DTT-reduced conditions before PE conjugation.
Concentration	25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Purity	>90%
Conjugation	PE
Buffer	1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
Preparation	Affinity Ni-NTA
Applications	FACS
Storage	An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
Stability	6 Months
Bioactivity	Positive The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
Reference Data
RefSeq	AAH09272.1
Locus ID	10232
UniProt ID	Q13421
Protein Families	FACS

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