Trim33 (NM_053170) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR227454 representing NM_053170 Red=Cloning site Green=Tags(s) MAENKGGGEAESGGGGSGSAPVTAGAAGPTAQEAEPPLAAVLVEEEEEEGGRAGAEGGAAGPDDGGVAAA SSSSAPAASVPAASVGSAVPGGAASTPAPAAAPAPAPAPAPAPAPAPAPAPGSSSGPPLGPPASLLDTCA VCQQSLQSRREAEPKLLPCLHSFCLRCLPEPERQLSVPIPGGSNGDVQQVGVIRCPVCRQECRQIDLVDN YFVKDTSEAPSSSDEKSEQVCTSCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESV GTSGQRPVFCPVHKQEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKKNY VHFAATQVQNRIKEVNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQMKLLQQQNDITGLS RQVKHVMNFTNWAIASGSSTALLYSKRLITFQLRHILKARCDPVPAANGAIRFHCDPTFWAKNVVNLGNL VIESKPAPGYTPNVVVGQVPPGTNHISKTPGQINLAQLRLQHMQQQVYAQKHQQLQQMRLQQPPAPIPTT TATTQQHPRQAAPQMLQQQPPRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSAPQYSMMQPH LQRQHSNPGHAGPFPVVSAHNPINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQ LEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSRGSCGSS GRTAEKSAHSFKSDQVKVKQEPGTEEEICSFSGAVKQEKTEDGRRSACMLSSPESSLTPPLSTNLHLESE LDTLTGLENHVKTEPTDISESCKQSGLSNLVNGKSPIRNLMHRSARIGGDGNSKDDDPNEDWCAVCQNGG DLLCCEKCPKVFHLTCHVPTLLSFPSGDWICTFCRDIGKPEVEYDCDNMQHSKKGKTAQGLSPVDQRKCE RLLLYLYCHELSIEFQEPVPVSIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRLIFKNCER FNEMMKVVQVYADTQEINLKGDSEVAKAGKAVALYFEDKLSEIYSDRTFTPLPEFEQDEDDGEVTEDSDE DFIQPRRKRLKSDERPVHIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	124.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_444400
Locus ID	94093
UniProt ID	Q99PP7, E9QP19
Refseq Size	8868
Cytogenetics	3 F2.2
Refseq ORF	3420
Synonyms	8030451N04Rik; AI413936; Ecto; Tif1g
Summary	Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).[UniProtKB/Swiss-Prot Function]

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