Eif4g1 (NM_145941) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR212048 representing NM_145941 Red=Cloning site Green=Tags(s) MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQGGFRSLQHFYPSRAQPPSSAA SRVQSAAPARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGA SPTEFGTYAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTA STPTPPQTGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPIL EPGSESNLGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFS SSPLQVSTALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPS PPAVDLSPVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPL DSTPVPAQLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSP ESEGSMVPTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFAS MQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGP AGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRS ILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVT VNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELF KLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIR FMLQDVLDLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPL VDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPA TLNRFSALQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSK EVEERSRERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMK EAVQCVQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDME IDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAG LSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNE QQIASNTLVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPAN LLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	176.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_666053
Locus ID	208643
UniProt ID	Q6NZJ6
Refseq Size	5481
Cytogenetics	16 B1
Refseq ORF	4800
Synonyms	E030015G23Rik; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; eIF4GI
Summary	This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]

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