Pan2 (NM_133992) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211796 protein sequence Red=Cloning site Green=Tags(s) MNFEGLDPGLAEFSPAMHSTLDPVLDAHLNPSLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA EVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGGDDIRQIQSLENGILFLTKNNLKYM ARGGLIIFDYLLDENEDMHSVLLTDNSTLLVGGLQNHVLEIDLNTVQETQKYAVETPGVTIMRQTNRFFF CGHTSGKVSLRDLRSFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITP LQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDS EGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDALLSDWPAANSAPAP RRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDHEFDNFSQVTESPTGREEEPLHTVSKKY RKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACELG FLFHMLDLSRGDPCQGSNFLRAFRTIPEASALGLILADSDEASGKGSLARLIQRWNRFILTQLHQDMQEL EVPQAYRGAGGSFCSSGDSIIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPEDKTGKNYDFAQVLK RSICLEQNTQAWCDNCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRLQAEVAFKIAVKKYGGEMK SKEFALADRKELRSPEGFLCSSIEELKNVWLPFSIRMKMTKNKGLDVCNWADEHELSSLGAPSQWGPARA EEELGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKYEAVQFDM NWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQVGDLVGLDAEF VTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLD AKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLA WYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPESQTSPK NAAVFSVLAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	135.3 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_598753
Locus ID	103135
UniProt ID	Q8BGF7
Refseq Size	4450
Cytogenetics	10 D3
Refseq ORF	3600
Synonyms	1200014O24Rik; AI047843; AW742773; mKIAA0710; Usp52
Summary	Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity).[UniProtKB/Swiss-Prot Function]

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