Zw10 (NM_012039) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR210603 protein sequence Red=Cloning site Green=Tags(s) MASFVTEVLAHSGSLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPTMQSAQALVTQVDTLSNDI DQLKSRIETEVCRDLHISTVEFTNLKQQLERDSVVLTLLKQLQEFSSAIEEYNSALAEKKYIPAARHLEE AQECLKLLKSRKCFDLKMLKSLSMELTVQKQNILYHLGEDWQKLVVWKFPPAKDTSSLESCLQTELHLCT EQPEKEDMTPLPSISSVLLAFSILGELPTKLKSFGQMLLKYILKPLVTCPSLHAVIERQPSSVSICFESL TTDLEHPSPPEAFAKIRLVLEVLQKQLLDLPLDADLEIGKVPGIVLAEMLGEGIWEDLSECLIRNCLVYS IPTNSSKLQEYEEIIQSTEEFEKFLKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIH NTVKIGPDCKEALPDLPSPDADHKLQVQTVCKAQFTDAGNLEPETSLDPQSFSLPTCRISEAVKKLMELA YQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLRKLPQLAAIHHNNCMYIAHHLLTLGHQFR LRLAPILCDGTTTFVDLVPGFRRLGTECFLAQMQAQKGELLERLSSARSFANMDDEENYSAASKAVRQVL HQLRRLGIVWQDVLPVNIYCKAMGTLLNTAIAEMMSRITALEDISTEDGDRLYSLCKTVMDEGPQVFAPL SDENKNKKYQEEVPVYVSKWMPFKELMIMLQASLQEIGDRWADGKGPLATAFPSSEVKALIRALFQNTER RAAALAKIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	88.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_036169
Locus ID	26951
UniProt ID	O54692
Refseq Size	2917
Cytogenetics	9 A5.3
Refseq ORF	2337
Synonyms	6330566F14Rik; MmZw10
Summary	Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity).[UniProtKB/Swiss-Prot Function]

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