Polk (BC052820) Mouse Recombinant Protein
CAT#: TP510382
Purified recombinant protein of Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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CNY 2900.00
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CNY 600.00
Specifications
| Product Data | |
| Species | Mouse |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>MR210382 protein sequence
Red=Cloning site Green=Tags(s) MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYEYDPNFMAMSLDEAYLNITQH LQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNP QIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIK DLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMS VERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAI AKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGGSLDKTAKTELAK PLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSQSFETSENSNDCQTFICPVCF REQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITSVDGV DLTGTEDRSLKAASMDTLENNRSKEECPDIPDKSCPISLANETISTLSRQESVQPCTDEVVTGRALVCPV CNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPRGATSPSAPP CLATMVPLMLPYLPVCISVSCEQMQLELAMMSMKNISLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-MYC/DDK |
| Predicted MW | 82.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| Locus ID | 27015 |
| UniProt ID | Q9QUG2 |
| Refseq Size | 3517 |
| Cytogenetics | 13 D1 |
| Refseq ORF | 2217 |
| Synonyms | Dinb1 |
| Summary | DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:12432099). Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
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