Mettl13 (NM_144877) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR210110 protein sequence Red=Cloning site Green=Tags(s) MNLLPKSSKEFGSADYWEKFFQQRGKTAFEWYGTYLELCEVLHKYIKPKEKVLVIGCGNSELSEQLYDVG YQDIVNIDISEVVIKQMKERNGSRRPHMSFLKMDMTQLEFPDATFQVVLDKGTLDAVLTDEEEVTLRQVD RMLAEVGRVLQVGGRYLCISLAQAHILKKAVGHFSREGWMVRAHQVASSQDRVSEAEPRFSLPVFAFVMT KFRPVPGSALQIFELCTQEQGKPVRLESADQLAEAVRERQYYAWLCSQLRRKAGLGSVSLDLCSGDTGEP RYTLHVVDNPAVKPSRDNHFAIFIIPQGRETEWLFGMEEGRKQLAASAGFRRLVTVALHRGQRYAGMESI QAELSARVMELAPAGLPPQQQVPFLSVGGDIGVRTVQHQDHSALSGDYVIEDVQGEDRWYFRRLIFLSNR NVVQSEARLLKDTSHRAQKKRKKDRKKQRPADTSEDFPPAPGQSIDKSYLCCEHHKAMVAGLALLRNPEL LLETPLTLLVVGLGGGSLPLFVHDHFPKSRIDAVEIDPTMLEVATQWFGFSQSDRMKVHIADGLDYITSL AGEAPPHYDVIMFDVDSKDPTLGMSCPPPAFVDQVFLQKVKSILCHDGVFILNLVCRDVRLKDSVLAGLK AAFPLLYVRRIEGEVNEILFCQLHPEQKLATPELLEMAQVLERTLRKPGQGWDDTYVLSDMLKTVKIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	78.8 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_659126
Locus ID	71449
UniProt ID	Q91YR5
Refseq Size	2801
Cytogenetics	1 H2.1
Refseq ORF	2094
Synonyms	5630401D24Rik
Summary	Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation. Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2'. Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55'.[UniProtKB/Swiss-Prot Function]

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