PUF60 (NM_078480) Human Recombinant Protein
CAT＃: TP323492
Recombinant protein of human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, 20 µg
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CNY 2900.00
CNY 6650.00
| Cited in 2 publications. |
Product images
CNY 600.00
Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>Peptide sequence encoded by RC223492
Blue=ORF Red=Cloning site Green=Tag(s) MATATIALVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKA KKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYE LGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNI GQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKA QSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLG TLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEP KKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVT EECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFD NSDLSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC223492 also available, TP323492 |
| Tag | C-Myc/DDK |
| Predicted MW | 59.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Bioactivity | WB positive control (PMID: 26253095) ELISA capture for autoantibodies (PMID: 29541951) |
| Reference Data | |
| RefSeq | NP_510965 |
| Locus ID | 22827 |
| UniProt ID | Q9UHX1 |
| Refseq Size | 1946 |
| Cytogenetics | 8q24.3 |
| Refseq ORF | 1674 |
| Synonyms | FIR; RoBPI; SIAHBP1; VRJS |
| Summary | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
| Protein Pathways | Spliceosome |
Citations (2)
| The use of this Proteins has been cited in the following citations: |
|---|
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The prevalence and clinical significance of anti-PUF60 antibodies in patients with idiopathic inflammatory myopathy
,Zhang, YM;Yang, HB;Shi, JL;Chen, H;Shu, XM;Lu, X;Wang, GC;Peng, QL;,
Clin. Rheumatol.
,PubMed ID 29541951
[PUF60]
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PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren's syndrome
,Fiorentino, DF;Presby, M;Baer, AN;Petri, M;Rieger, KE;Soloski, M;Rosen, A;Mammen, AL;Christopher-Stine, L;Casciola-Rosen, L;,
Ann. Rheum. Dis.
,PubMed ID 26253095
[PUF60]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC409189 | PUF60 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1470.00 |
|
| LC415385 | PUF60 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY409189 | Transient overexpression lysate of poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 |
CNY 4840.00 |
|
| LY415385 | Transient overexpression lysate of poly-U binding splicing factor 60KDa (PUF60), transcript variant 2 |
CNY 3080.00 |
|
| PH303454 | PUF60 MS Standard C13 and N15-labeled recombinant protein (NP_055096) |
CNY 19520.00 |
|
| PH323492 | PUF60 MS Standard C13 and N15-labeled recombinant protein (NP_510965) |
CNY 19520.00 |
|
| TP303454 | Recombinant protein of human poly-U binding splicing factor 60KDa (PUF60), transcript variant 2, 20 µg |
CNY 2900.00 |
