UBE2D1 / SFT (1-147, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MGSMALKRIQ KELSDLQRDP PAHCSAGPVG DDLFHWQATI MGPPDSAYQG GVFFLTVHFP TDYPFKPPKI AFTTKIYHPN INSNGSICLD ILRSQWSPAL TVSKVLLSIC SLLCDPNPDD PLVPDIAQIY KSDKEKYNRH AREWTQKYAM
Tag	His-Tag
Predicted MW	19 kDa (170aa) confirmed by MALDI-TOF
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant human UBE2D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_003329
Locus ID	7321
UniProt ID	P51668
Cytogenetics	10q21.1
Synonyms	Ubiquitin conjugating enzyme E2 D1, SFT, Stimulator of Fe transport, Ubiquitin-conjugating enzyme E2D 1, "UBC4/5 homolog, yeast", UbcH5A, UBCH5, UBC4/5, E2(17)KB1
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Protein Pathways	Ubiquitin mediated proteolysis

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