Cytidine deaminase (1-146, His-Tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ
Tag	His-Tag
Predicted MW	18.3 kDa (166aa) confirmed by MALDI-TOF
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 90% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 100mM NaCl, 40% glycerol
Preparation	Liquid purified protein
Applications	SDS-PAGE, Enzyme Activity
Protein Description	Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific activity is > 10,000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C.
Reference Data
RefSeq	AAH54036.1
Locus ID	978
UniProt ID	P32320
Cytogenetics	1p36.12
Synonyms	Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase
Summary	This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. [provided by RefSeq, Jul 2008]
Protein Families	Stem cell - Pluripotency
Protein Pathways	Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism

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