Cytokeratin 14 Human Protein

CAT#: AR09363PU-N

Cytokeratin 14 human recombinant protein, 0.25 mg

Size: 100 ug 250 ug



Need it in bulk or customized?
Get a free quote

CNY 12,330.00


货期*
7周

规格
    • 250 ug

Product images

Specifications

Product Data
Species Human
Expression Host E. coli
Concentration lot specific
Purity >95% determined by SDS gelelectrophoresis
Buffer Presentation State: Purified
State: Lyophilized purified protein
Preparation Lyophilized purified protein
Applications Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
Protein Description Recombinant human keratin K14 (formerly also designated cytokeratin 14). 
Molecular weight: 
51,662 (+ methionin).
51,530 (- methionin; calculated from sequence).
50,000 (determined by SDS gelelectrophoresis).
Note Isoelectric Point: pI 5.3 (4.92 calculated from sequence)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_000517
Locus ID 3861
UniProt ID P02533
Cytogenetics 17q21.2
Synonyms CK14; EBS3; EBS4; K14; NFJ
Summary This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Customer Reviews 
Loading...