IFNA2 / Interferon alpha-2 (24-188) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
Predicted MW	19.4 kDa (166aa) confirmed by MALDI-TOF
Concentration	0.5mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. Phosphate-Buffered Saline (pH 7.4), 10% glycerol
Preparation	Liquid purified protein
Applications	SDS-PAGE, Bioactivity
Protein Description	Interferon alpha-2b was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Interferon alpha−2b was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Bioactivity	Measured by its binding ability in a functional ELISA with Human IFN-alpha/beta R2 (CAT# ATGP3952) in the presence of Human IFN-alpha/beta R1 (CAT# ATGP3894).
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_000596.2
Locus ID	3440
UniProt ID	P01563
Cytogenetics	9p21.3
Synonyms	Interferon alpha 2, IFN-alpha-2, Interferon alpha-A, IFNA, IFN-alphaA, LeIF A, IFNA2A, IFNA2B, IFNA2C, alpha-2a interferon, Interferon alpha 2b
Summary	This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach. [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Antigen processing and presentation, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of autophagy, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway

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