PCNA (1-261) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
Predicted MW	28.7 kDa (261aa) confirmed by MALDI-TOF
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 20% glycerol
Preparation	Liquid purified protein
Applications	SDS-PAGE
Protein Description	Recombinant Human PCNA, was expressed in E.coli and purified by conventional chromatography techniques.
Note	NCBI Accession No.: NP_002583
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_002583.1
Locus ID	5111
UniProt ID	P12004
Cytogenetics	20p12.3
Synonyms	Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA
Summary	The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

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