DPM3 (NM_018973) Human Mass Spec Standard

Specifications

Product Data
Description	DPM3 MS Standard C13 and N15-labeled recombinant protein (NP_061846)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC212952
Predicted MW	13.3 kDa
Protein Sequence	Sequence Link (showhide) >RC212952 protein sequence Red=Cloning site Green=Tags(s) MLSVGGLRLSLVRFSFLLLRGALLPSLAVTMTKLAQWLWGLAILGSTWVALTTGALGLELPLSCQEVLWP LPAYLLVSAGCYALGTVGYRVATFHDCEDAARELQSQIQEARADLARRGLRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_061846
RefSeq Size	532
RefSeq ORF	366
Synonyms	CDG1O; MDDGB15; MDDGC15
Locus ID	54344
Cytogenetics	1q22
Summary	Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex. [provided by RefSeq, Jul 2008]
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis

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