HIBCH (NM_014362) Human Mass Spec Standard

Specifications

Product Data
Description	HIBCH MS Standard C13 and N15-labeled recombinant protein (NP_055177)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC209814
Predicted MW	43.4 kDa
Protein Sequence	Sequence Link (showhide) >RC209814 protein sequence Red=Cloning site Green=Tags(s) MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLGKKGCTGVITLNRPKFLNALTLNMIRQIYP QLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIH GITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRA GIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEI IENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVL IDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_055177
RefSeq Size	1958
RefSeq ORF	1158
Synonyms	HIBYLCOAH
Locus ID	26275
Cytogenetics	2q32.2
Summary	This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Protein Pathways	beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation

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