MASA (ENOPH1) (NM_021204) Human Mass Spec Standard

Specifications

Product Data
Description	ENOPH1 MS Standard C13 and N15-labeled recombinant protein (NP_067027)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC209510
Predicted MW	28.9 kDa
Protein Sequence	Sequence Link (showhide) >RC209510 protein sequence Red=Cloning site Green=Tags(s) MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLD GAVPIPAASGNGVDDLQQMIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVR KWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFL TDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_067027
RefSeq Size	2191
RefSeq ORF	783
Synonyms	E1; MASA; MST145; mtnC
Locus ID	58478
Cytogenetics	4q21.22
Summary	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).[UniProtKB/Swiss-Prot Function]
Protein Pathways	Cysteine and methionine metabolism

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