PIGL (NM_004278) Human Mass Spec Standard

Specifications

Product Data
Description	PIGL MS Standard C13 and N15-labeled recombinant protein (NP_004269)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC209339
Predicted MW	28.5 kDa
Protein Sequence	Sequence Link (showhide) >RC209339 protein sequence Red=Cloning site Green=Tags(s) MEAMWLLCVALAVLAWGFLWVWDSSERMKSREQGGRLGAESRTLLVIAHPDDEAMFFAPTVLGLARLRHW VYLLCFSAGNYYNQGETRKKELLQSCDVLGIPLSSVMIIDNRDFPDDPGMQWDTEHVARVLLQHIEVNGI NLVVTFDAGGVSGHSNHIALYAAVRALHSEGKLPKGCSVLTLQSVNVLRKYISLLDLPLSLLHTQDVLFV LNSKEVAQAKKAMSCHRSQLLWFRRLYIIFSRYMRINSLSFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_004269
RefSeq Size	1163
RefSeq ORF	756
Synonyms	CHIME
Locus ID	9487
Cytogenetics	17p11.2
Summary	This gene encodes an enzyme that catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which is the de-N-acetylation of N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI). Study of a similar rat enzyme suggests that this protein localizes to the endoplasmic reticulum. [provided by RefSeq, Jul 2008]
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways

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