Nup53 (NUP35) (NM_138285) Human Mass Spec Standard

Specifications

Product Data
Description	NUP35 MS Standard C13 and N15-labeled recombinant protein (NP_612142)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC208891
Predicted MW	34.8 kDa
Protein Sequence	Sequence Link (showhide) >RC208891 protein sequence Red=Cloning site Green=Tags(s) MAAFAVEPQGPALGSEPMMLGSPTSPKPGVNAQFLPGFLMGDLPAPVTPQPRSISGPSVGVMEMRSPLLA GGSPPQPVVPAHKDKSGAPPVRSIYDDISSPGLGSTPLTSRRQPNISVMQSPLVGVTSTPGTGQSMFSPA SIGQPRKTTLSPAQLDPFYTQGDSLTSEDHLDDSWVTVFGFPQASASYILLQFAQYGNILKHVMSNTGNW MHIRYQSKLQARKALSKDGRIFGESIMIGVKPCIDKSVMESSDRCALSSPSLAFTPPIKTLGTPTQPGST PRISTMRPLATAYKASTSDYQVISDRQTPKKDESLVSKAMEYMFGW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_612142
RefSeq Size	1637
RefSeq ORF	978
Synonyms	MP-44; MP44; NP44; NUP53
Locus ID	129401
Cytogenetics	2q32.1
Summary	This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013]

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