EIF4E (NM_001968) Human Mass Spec Standard

Specifications

Product Data
Description	EIF4E MS Standard C13 and N15-labeled recombinant protein (NP_001959)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC207333
Predicted MW	25.1 kDa
Protein Sequence	Sequence Link (showhide) >RC207333 protein sequence Red=Cloning site Green=Tags(s) MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST TKNRFVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_001959
RefSeq Size	4749
RefSeq ORF	651
Synonyms	AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F
Locus ID	1977
Cytogenetics	4q23
Summary	The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway

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