LAPTM4B (NM_018407) Human Mass Spec Standard

Specifications

Product Data
Description	LAPTM4B MS Standard C13 and N15-labeled recombinant protein (NP_060877)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC207325
Predicted MW	35 kDa
Protein Sequence	Sequence Link (showhide) >RC207325 protein sequence Red=Cloning site Green=Tags(s) MTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGL QARRSTLLKTCARASATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALAD PDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICATATYGAYKQRAAWIIPFFCYQIFDFALNMLVA ITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSS DVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_060877
RefSeq Size	2242
RefSeq ORF	951
Synonyms	LAPTM4beta; LC27
Locus ID	55353
Cytogenetics	8q22.1
Summary	Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).[UniProtKB/Swiss-Prot Function]
Protein Families	Transmembrane
Protein Pathways	Lysosome

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