MED31 (NM_016060) Human Mass Spec Standard

Specifications

Product Data
Description	MED31 MS Standard C13 and N15-labeled recombinant protein (NP_057144)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC203047
Predicted MW	15.8 kDa
Protein Sequence	Sequence Link (showhide) >RC203047 protein sequence Red=Cloning site Green=Tags(s) MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYP QCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_057144
RefSeq Size	1656
RefSeq ORF	393
Synonyms	3110004H13Rik; CGI-125; Soh1
Locus ID	51003
Cytogenetics	17p13.1
Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[UniProtKB/Swiss-Prot Function]
Protein Families	Transcription Factors

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