RFK (NM_018339) Human Mass Spec Standard

Specifications

Product Data
Description	RFK MS Standard C13 and N15-labeled recombinant protein (NP_060809)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC202812
Predicted MW	18.4 kDa
Protein Sequence	Sequence Link (showhide) >RC202812 protein sequence Red=Cloning site Green=Tags(s) MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVV SIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELP EHLKIKEDNFFQVSKSKIMNGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_060809
RefSeq Size	2707
RefSeq ORF	486
Synonyms	RIFK
Locus ID	55312
Cytogenetics	9q21.13
Summary	Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Riboflavin metabolism

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