DDOST (NM_005216) Human Mass Spec Standard

Specifications

Product Data
Description	DDOST MS Standard C13 and N15-labeled recombinant protein (NP_005207)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200672
Predicted MW	50.7 kDa
Protein Sequence	Sequence Link (showhide) >RC200672 protein sequence Red=Cloning site Green=Tags(s) MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCASGPRTLVLLDNLNVRETHSLFFRSLKDR GFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRE LGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPL VLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGS QRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDG DDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYER FIPSAYPYYASAFSMMLGLFIFSIVFLHMKEKEKSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_005207
RefSeq Size	2144
RefSeq ORF	1368
Synonyms	AGER1; CDG1R; GATD6; OKSWcl45; OST; OST48; WBP1
Locus ID	1650
Cytogenetics	1p36.12
Summary	This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis

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