Neo1 Mouse Gene Knockout Kit (CRISPR)

pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC CTTCCTCTTC CAGCCCTTCC TCTTTCACTG ACTGACTGAC TGGAAGACAC ACCTTCCGGG GTTTCCCTGA CGCCAGCAGC AGCAGCAGAG CCCGGCGCGC GCGCCCCGCG CCCGTGCCCG TCTCCCGAGA GCCAGAGGGG GGAGGGGAGC GGGCCCGCCG CCTGCCCCTG CGCCAGCTCG GCTCCACCGC CGCGGCTGCC CGCGAAGCGA CCGCCGGGAT TGGAGCCCGG CAGGCCGCGG GGCGCGAAGA GGGTGCTCGT GGGGAGGGAG GCGCGCGTGC GCGGCTGTGA GCGTGCGCGG CACGGCGCGC TCCGGCGCCC GCCCCCCACC CCGCGGTCTC CGCCCCCCTC GCTCTACCGT GAGGAGCCCG AGTCGGCGGC GGGTGGCGGC GCCTGGAACC TGGAGAGACC GAGCCACCCC CCGGCTCTCG GCCGGAATGT ACTGATTCTC CTCTGCTCTC CTCCCCGCCC CGCTGCAGGA GGGAGGCGCC CGGAGTCTTT CCCCCTGGGC GCGCGAGGGG GCCGCGCGGG CCGGGCCGGG CCGGGCTGGA GCCGAGCCCT GCGGCGCAGA GACCGGCTGA GGCGCGCTGA GGGAAGGGCG CGAGCGCTCC GCGGCGCTAT CGCCGCCGCC GCCGCCGCCA CTCGTGGGGT AGAGTTGCTG CCGCTGCTGC TGCTGCTCGG ACGCCCGGCG TCCGGCGCCG CGGCCACGAA GAGCGGCTCC CCGCCGCAGT CCGCAGGTAA GTCGGGCCGG GCCCGGGACG CCGTGGGGGC TGGGGCGCGG CACCGGCTCC CGTGGCCGCT GCGGTGGGTT TTTGTTGTTG TTTAGGATCC AGAGGGGAAC GTGCGGGAAC TTTTCCTCCT CGGCTCCCGT TGCTGGAGAG AAAAGGGTCT CGCGTGGTGG AAAGCGGAGC AGGCAGGAGG GGTTCCTACC GGGGGAGGGG CGCCTCCGGG ATCCGGGCCG GAGCTCTGGG GTCCAGTTCT GCGCTTTGTA CCGGGAGGCA GGTTCACCCC TCGCCTCTTT CCCCTCCCCC AGGCTCATCC CACTTGTAAG AGTGGGACAT TCTTGTGCTC ACACAAGCAC CAGTAGAGGC CACTACACAC CTCATTAGAT AAACAATCGT ACAGCCAATT CATTAATTTT CAGGAAGGGA TGGAGGAGGG GGCTTGAATT CATTCTCTTC ACCCTCCATC CCCCTCGGCC ATGGGTTGCC CAGCTTCTGA AGTCCAGGGA AGCGCTGTCG GATCTCGAGG GACTTCCACA GTCTTCACTG ACTGACTGAC TGGAAAGTCC TCTCCACTGA CTGTAGCCTC CAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCAACGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGACCC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTT