Mcam Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Mcam
Locus ID	84004
Kit Components	KN309859G1, Mcam gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAACATAAAGAACTCACCCG KN309859G2, Mcam gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGATCTCCGGTACACTCAGG KN309859-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAGGCACCGT TTCCAACTCT CCCGCCCCCT TGCAGGCACC CCTAGGTCTG TGGGACCTGG CACAGTTCTT CCTCCAAGGC TGCCAGGCCA GCGAAGACTC TGGAACAGTG CCAAGCCGGC AGCCGCCGCA GTGAAGCGGT CATTCCAGAA GGCGACCAGG GCCAGCTCCA TGCCTCCACA GCAGGGTAGA GGAACCTGAC TGAGAGTCCT CTATCAGGGA TACCTCTCTG TTGCTCCTCT GCTAGAGGGT CCCCGCTAGT AGTGGACAAA CCTGAGCGAG CGCTGCTACT GCGTCCAAGA GTCTACAGCG ACCGGGCCAG CTTCTGCCGC CTCGGCCAGT CATTCGAGCC CCAGGACACC TGTCATTGCT CCTTCAACCT TTCCAAGCCA GAAAACGGGA GTTAGCCTCT AGGCCTGGAT CGGGGAGCAG CAGCTCGCAG CCAGCGCGCC GGGGGCCCGC CCCCTAGGGC GCGGGGAGGG GGCGGGGGGC TACGCGGGGC GGGGCCGGGC GGCGCCCGGG GTCCCCTCCC CCGCCCCCCT GACGTCAGCC CCCGACAGCG GCGAGTTCCT CACTTGGCTC GCGCCCTCCG TCGGGGAAGC TCGGGGCTCT GCACCCCAGT GGGCGCTCCT TCTTGCCAGA GTCCCCGATT TCCAGGCGCC CCGCGTGCGT GCAACTTCTT TGCGGGTGTG CAGCCTAGGT TTTAGAGTAT TCTGGGAACG GACTAGTCGG GTTGCTGCAG CAGCGAGCGG GAAGGGGTGG GGCCGAGCTT CTGCAGGACG CTGGGCCCGG CCTCCCGCAG GTGTTCGCTC TGGGTGCTGG ATGCTGGGTG CTGGAGTCCC GGCAGAGAGG CGCAGCCGGG GCGCATCCCC AACCCGTGCA TCCCGCGCCC GCCTGTTGCT GGAAAAGGCA GTTTCCCTAG CTGGAGGGGG CCGCCGACCC CTCCCCCTCC GCCTGGGCCT CACGCCAGTT TGGAGGTGAG CGAGGGAGGA GGCCGCTCCT GCTGGGGCTG GGCGTTGGCA AGGGCAGCGG GTGGTGTGGG TCTCCTTGCG CCTCCGGGTT TGACGGGATT ACAGGGGAAA GACAACAGAT GGAGAAGTTC TACAACAGTA ACAGCTTCTG TGGTCCACTA GAGTCAGGTG GAGAGAGCCT GGCACCTCGG AGCTTTCCTT TAAGCCATCG AGTAGACATG CTTTCCTGGG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_023061, NM_001359530
Synonyms	1-gicerin; AV025631; CD146; CD149; Muc18; s-endo; s-gicerin
Summary	Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).[UniProtKB/Swiss-Prot Function]

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