B Raf (BRAF) Human Gene Knockout Kit (CRISPR)

CAT#: KN211013BN

BRAF - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

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CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


BRAF mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


BRAF (Myc-DDK-tagged)-Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
    • 10 ug

CNY 8,416.00

Specifications

Product Data
Format 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA mBFP-Neo
Symbol B Raf
Locus ID 673
Kit Components

KN211013G1, B Raf gRNA vector 1 in pCas-Guide CRISPR vector

KN211013G2, B Raf gRNA vector 2 in pCas-Guide CRISPR vector

KN211013BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_004333, NM_001354609, NR_148928
Synonyms B-raf; B-RAF1; BRAF1; NS7; RAFB1
Summary This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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