CD147 (BSG) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CD147
Locus ID	682
Kit Components	KN203894G1, CD147 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GATATTTGGGGGCGGCGAGT KN203894G2, CD147 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGGACGCGTCTCCCCAAGAA KN203894-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GCAGGAGAAT CGCTTGAACC CGGGAGGCGG AGGTTGCAGA GAGTCGAGAT CGCGCCGCTG CACTCCAGCC TGGGTGACAC AGCGAGACTC CAACACACAC ACACACAAAA GATATGGCGC GTCTCATTCA TTCACAAGTT CCTCTTCAAA GGTTTGGCTC GTTCAGCGCC CTCGTTCTTC GTTTCCTACT TCCAAGGCCA ACCTGACTTC CTCACCCTCT GGGCCTCCCC GTCTGAGTCT CAGTAAACAA GTTTCCCGCC GGTCCTGATC TGCAGAGATG GCACCTGCCA CCCACTCTGC ACATCCCCCT CCCGTCGCCA CGGGCTCTTC CCGCCAGTGT AGCCACATTC CTGCCCCTTT CCAGTTAGCC CTTCGCGTTC GGCTTAGTCT GCGGTCCTCT TGCATTGCGA CTCCGAGTTT AACTTCCAAC ACACACTTTC AACCTCCAAG AGACGCCCCC ACCTGTGTCG CCCCAATAGC GACTTTTCTC ACCGTGGTCG CCGCGGAACT TCAAGGGTCC TTCCTACCCG CGTTGCTGAG AGTCTGGGTT TACGCGTCAC CTCGGGCGGG ACCCGATCCT CCGCTCCTGA GGCCCCCACA TCCCGCAAAG AGAAACCAGC ACTTAGAAAA TTTCTCAGCG AGACCACTTT ACTTGTACGG AAGGGGGCTG CCTGCGTGGG ATGCAATCTC CAGAGCACAC GGGACAAAGG AAGGGAGCTT CTGTTCCTCT GTCCTTTCCC TGTTGGCTGG GGTTGGACGC CCACAATCTA AGCTGATCCC GGTTGGCTAA AACTTAAAAC TTTTTTAAAA TAGGGTAAAC GCGCCACCTG CGAGGAAAGA AGAAGGGGGT AAGGTTGATT GACAACTTTA GACCTGGAAG TTGAGTTTCT GAAGAGGAAC TTACTTGTCC CAACCAAATG GTGCACAGGA CGTTTATACT AAGAAATTAT TCATTTTCTG AGATCCAGAT TCAAGTGCCC TTTAAGTATT TTTATTTGGG CGGAGGAGGT CACTTCCTCC CACCTAATTT ATTAATACGT TCATATATAT ATATATATAT GTACATTTTT AAAGGCAGAG ATGTGCGTCT CGCCATGTTG CCGCGTCTGG TCTCGAGCTC CCGGGCTCAA GCGATCTTCC CGCCTCGACC TCCCAAGGCT CTGGGAGTAC AGACGTGAGC CACAGCGGCC GGCCCGTGTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001322243, NM_001728, NM_198589, NM_198590, NM_198591
Synonyms	5F7; CD147; EMMPRIN; OK; TCSF
Summary	The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]

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