WDR79 (WRAP53) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	WDR79
Locus ID	55135
Kit Components	KN200142G1, WDR79 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCCGCATTTTTATTCATCG KN200142G2, WDR79 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGACTGCTGTCCTTCAGACC KN200142-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GTTGTATTCC TGAGTGCCTA TATCAGTGCT GGGTAGCAAG TAAGAGCTCG ATAATAAATA TTTTTTGAAT GAGGGAGACA GGTCTGAAGC CTGGAGAATG AGATGCAGAA GAGGTGCAAG ACCTGCTGCG CCCTCTGCAG GCGGCGGGGG GGCGGTGCAG GTGCTTTAAG AATTACCGCG GGACTCGGTA GGGGGAGCGT AGGCGCTTCT CGCCAAGATA GAAGCGTTCA GACTACAACT CCCAGCAGCC ACGAGGAGCC CTAGGGCTTG ATGGGAACGG GAAACCTTCT AACCTTTCAC GTCCCGGCTC CGCGGGTTCC GTGGGTCGCC CGCGAAATCT GATCCGGGAT GCGGCGGCCC AATCGGAAGG TGGACCGAAA TCCCGCGACA GCAAGAGGCC CGTAGCGACC CGCGGTGCTA AGGAACACAG TGCTTTCAAA AGAATTGGCG TCCGCTGTTC GCCTCTCCTC CCGGGAGTCT TCTGCCTACT CCCAGAAGAG GAGGGAAGCA CAGGTGGGTT TCTTTAGCTC TGCGTCGGAT CCCTGAGAAC TTCGAAGCCA TCCTGGCTGA GGCTAATCTC CGCTGTGCTT CCTCTGCAGT CCGCCTCCCG AAAGGGGGGA TCCGCCCCGG TTGTCCCCAG ATCCTGTGGC TGGCTCAGCT GTGTCCCAGG AGCTACGGGA GGGGGACCCA GTTTCTCTCT CCACTCCCCT GGAAACAGAG TTTGGTTCCC CTAGTGAGTT GAGTCCTCGA ATCGAGGAGC AAGAACTTTC TGAAAATACA AGCCTTCCTG CAGAAGAAGC AAACGGGAGC CTTTCTGAAG AAGAAGCGAA CGGGCCAGAG TTGGGGTCTG GAAAAGCCAT GGAAGATACC TCTGGGGAAC CCGCTGCAGA GGACGAGGGA GACACGTAAG TGGTGATGGC AGTGGAGTGT GGAGTCTGGG GAGATGAAGT GTGAGGTCGA TCTGTCCTCT GGTCCTGAGA CCCACTTCTC CAGGCCTCTG CCCCCTTTGC TTGGCGACCC AGGTTTATTG TCCCCCATCT TCCTTTCAGC GCTTGGAACT ACAGCTTCTC CCAGCTGCCT CGATTTCTCA GTGGTTCCTG GTCAGAGTTC AGCACCCAAC CTGAGAACTT CTTGAAAGGC TGTAAGTGGT AAGGATAACA ACGGGGCAGG GAGCTGACCA CCCCCGAGAT TTTCACTGTA GE100003, scramble sequence in pCas-Guide vector
Reference Data
RefSeq	NM_001143990, NM_001143991, NM_001143992, NM_018081
Synonyms	DKCB3; TCAB1; WDR79
Summary	This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq, Mar 2011]

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